Fig 1.
Collection of sperm sample on a gauze pad whit massage technique base on digital manipulation.
Fig 2.
Differentiating between live (white) and dead (pink) chinchilla spermatozoa in a stained smear using aniline blue and eosin.
Table 1.
Seasonal distribution of successful semen collection.
Fig 3.
Seasonal variations in sperm concentration during the study period.
Values in parenthesis represent the number of samples evaluated per season. Different letters indicate statistical differences for sperm concentration. a;b;c: p≤0,05.
Table 2.
Seasonal distribution of motility parameters.
Fig 4.
Seasonal distribution ratio of live, intact and live, abnormal spermatozoa in eosin-aniline blue-stained smears.
Different letters indicate statistical differences for live, intact cells and abnormal cells by season. a;b;c: p≤0,05.
Fig 5.
Detailed seasonal distribution of morphological abnormalities in eosin-aniline stained smears.
Morphological abnormalities are plotted as a proportion of all examined cells. Different letters indicate statistical differences for different abnormalities by season. a;b;c: p≤0,05.